Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNHFTFQKLNGITPYIWTIFFILPFYFIWKSSSTFVIIVGIILTLLFFSVYRFAFVSKGWTIYLWGFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYVHLISAAVAANFSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAELVKLEERQRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNEVRKIVSSMK-----------GIRLKDELINIKQI----LEAA-DIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQA---------KTCRVDIQQLWKEVVITVSDDGTFKGEEN------------SFSKGHGLLGMRERLEFANGSLHI-DT-ENGTKLTMAIPNNSK 4E01 Chain:A ((146-404)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIE-DEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTA--GFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHID-

General information: TITO was launched using: RESULT: Template: 4E01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 603 -1436 -2.38 -7.48 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -2.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_4E01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E01-query.scw PDB file : Tito_Scwrl_4E01.pdb: