Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDYSQFPRKNILCVDMKSFYASVSAVTMGLNPLTCYLAVVGNTDRQGSVVLAASPALKKDFGIKTGSRLFEIPEDP-RIHIVNPQMKLFIRVSTEITKLFYRFVPEKCVHTYSIDESFLDAGK------EDPEEMAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNKLWKVRPLSKMWGIGGRMERNLNRMGISTIGQLAKFPLELLEKKFGI-MGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILLRDYTRSEEIKAVLLEICEEVARRARTHNKVGRTISLGIGYSKDELGGGFHRSKTIDLPTSITMDIYRCCLMLFNKFYS--GKTVRSVSVTLSNIEDDVNQQLSLFEVDNEKRRKLGFVMDGIRSKYGSKAILRAVSYTPAGTALQRAGLTGGHKS 4F50 Chain:A ((2-342)) ----------IVIFVDFDYFFAQVEEVLN-PQYKGKPLVVCVYS---SGAVATANYEARK-LGVKAGMPIIKAMQIAPSAIYVPMRKPIYEAFSNRIMNLLNKHAD--KIEVASIDEAYLDVTNKVEGNFENGIELARKIKQEILEKEKITVTVGVAPNKILAKIIADKSKP---NGLGVIRPTEVQDFLNE-LDIDEIPGIGSVLARRLNELGIQKLRDILSKNYNELEKITGKAKALYLLKLAQNKYSEPVENK--S-KIPHGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKR--IPKAIHVVAVTEDL---DIVSRGRTFPHGISK-ETAYSESVKLLQKILEEDERKIRRIGVRFSKFI------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4F50.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1505 -22625 -15.03 -69.40 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -15.03 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_4F50.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F50-query.scw PDB file : Tito_Scwrl_4F50.pdb: