Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MERRIFIRLRHRVLAHPGDIITVGD-------------------------------AAQIEGQLQLKKKLSAMPLYQVSEKDKNIVILDIIQVLRAIHLQDPTIDVQTVG---------------GAETIVEIQY----RKRNLSTVLFIGVWLLLFIG-----SCLAIMNFHEDVSMRDV------------------------HIALYEIITGERNDYPYLLQIPYSIGLGLGMIVFFNHIFKKRLNEEPSPLEVEMFNYQLDLDQYVAMHENQETIKDLHDR 5FN3 Chain:B ((78-467)) GAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVTDLVAVLCNETLFPALIYGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFY-FATDYLVQPFMDQLAFHQFYI-

General information: TITO was launched using: RESULT: Template: 5FN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 600 -82065 -136.77 -402.28 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -136.77 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.195

(partial model without unconserved sides chains): PDB file : Tito_5FN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN3-query.scw PDB file : Tito_Scwrl_5FN3.pdb: