Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------MEKFVEKMLGQALRQYGRNVAIDPLSPYEKQSLKAALQERRNEEPDEDLHAHIEDIIYDYVTNQGLFS-------------- 2TMV Chain:P ((1-154)) SYSITTPSQFVFLSSAWADPIELINLCTNALGNQFQTQQARTVVQRQFSEVWKPSPQVTVRFPDSDFKVYRYNAVLDPLVTALLGAFDTRNRIIEVENQANPTTAETLDA---TRRVDDATVAIRSAINNLIVELIRGTGSYNRSSFESSSGLVWTS

General information: TITO was launched using: RESULT: Template: 2TMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 119 9316 78.29 143.32 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain P : 0.57 3D Compatibility (PKB) : 78.29 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_2TMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2TMV-query.scw PDB file : Tito_Scwrl_2TMV.pdb: