Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAI---------QSLLNGLPKTKRHFITTAMEHQSIH-NCAAFLEQQGYDVTVIEPNEY-GLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHNKG----------ILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLT---THEYGFRAGTVNVPGIGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIR-SLPVTLAADTSKAECLPHIIGCFF----HSFEGQYVMLECNRSNICISTGSACSAGYHG-PSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA 3A9Z Chain:B ((20-430)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQ--------TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNT--CNFSIQGSQLRGYMVLAQCQ--TLLASVGASCHSDHEDRPSPVLLSCGIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQLEG----------------

General information: TITO was launched using: RESULT: Template: 3A9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2069 -18179 -8.79 -49.81 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -8.79 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3A9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9Z-query.scw PDB file : Tito_Scwrl_3A9Z.pdb: