Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------MKKIVGALAVFVITYALFSAAGYLFPVDQ---------EWYNSLKKPDWTPSGTAIGII--WAI------LFALISLSAAIV-------YAAFSFK-GAKSFWFTLLINYVLNQAFSYFQFTQKNLLAA-----------SLDCLLVAITAIVLLIIAKKYSRAASYLLLPY-FLWSAFATFLSFTINSMNL 3EPZ Chain:A ((18-267)) GPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIPGGVNGKNLGPINEWWITGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDQPIFLTPCMRDLIKLAGVTLGQRRAQA-

General information: TITO was launched using: RESULT: Template: 3EPZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 689 -62281 -90.39 -404.42 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -90.39 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_3EPZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EPZ-query.scw PDB file : Tito_Scwrl_3EPZ.pdb: