Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLWELFVAAILGIVEGLTEYAPVSSTGHMIIVDD----IWLKSSNLMSE---EAANSFKVVIQLGSILAVAIVFKDRILNLLGLKKNITSDQEQGHKLSIAQIAVGLVPAAVLGFLFEDYIDEYLFSVKTVAIGLIAGAILMLFADWVNKRKTATDTLDRISYKQAIAVGLFQCLSLWPGFSRSGSTISGGVILGLNHRAAADFTFIMAMPIMMGASFLSLVKHWDSLSSDLMPFFIVGFICAFVVALFVVRFFLRLINKIKLVPFA------IYRIILGVI--LLLIMM 5B02 Chain:A ((-5-258)) -----KMLAAEAANRDHVTRCVAQTGGSPDLVAHTAALRLYLRVPHFLTEWTTDPDRRAAVSRALALDIVSMKLLDDLMDDDTGLD-----------RVELACVCLRLHLRALHELESL------ARDPKAVTDILEQDAVHLCGGQIRTKRSRATN---LREWRAHASTYGSTFLGRYGALAAACGGEGQ------PADSVREFAEAFAMTITMADDLTDYDRNGER-DGNLAHLMRTGAVAGQDVVDLLEELRGRALAAVAAPPGAPGLVPVVHLYTDDVLVRLLPRHL

General information: TITO was launched using: RESULT: Template: 5B02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1112 -52204 -46.95 -213.95 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -46.95 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_5B02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B02-query.scw PDB file : Tito_Scwrl_5B02.pdb: