Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINVLIVEDDPMVGELNKRYL-SQIDGFQLKGI-ASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKSTLKLIWSSIQSFENESFTTEDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL 1ZH2 Chain:B ((1-112)) MTNVLIVEDEQAI----RRFLRTALEGDGMRVFEAETLQRGLLEAATRKPDLIILDLGLPDGDGIEFIRDLR-QWSAVPVIVLSARSEESDKIAALDAGADDYLSKPFGIGELQARL------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZH2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 520 -14134 -27.18 -128.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -27.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.682

(partial model without unconserved sides chains): PDB file : Tito_1ZH2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZH2-query.scw PDB file : Tito_Scwrl_1ZH2.pdb: