Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEYGFKDDSLSLHTDLYQINMAETYWRDGIHEKKAIFELFFRRLPFENGYAVF-AGLEKAIEYLENFKFTDSDLSYLQDELGYHEDFIEYLRGLSFT-GSL-YSMKEGELVFNNEPIMRVEAPLVEAQLIETALLNIVNY------QTLIATKAA---------RIKGV-I----GDEVALEFGTRRAHEMDA--AMWGARAALIGGFSATSNVRAGKRFNIPVSGTHAHALVQAYR--------DEYTAFKKYAETH-K-DCVFLVDTYDTLRSGMPNAIRVAKEFGDRINFIGIRLDSGDLAYLSKKARKMLDEAGFTDAK--VIASSDLDEHTIMNLKA--QGARIDVWGVGTKLITA-YDQPALGAVYKLVAIEEDGKMVDTIKISSNPEKVTT--PGRKKVYRIINQSNHHSEGDYIALYDEQVNDQKRLRMFHPVHTFISKFVTNFYAKDLHELIFEKGILCYQNPEISDIQQYVQDNLSLLWEEYKRISKPEEYPVDLSEDCWSNKMQRIHEVKSRIEEELEEE 3OS4 Chain:A ((8-388)) -------ILTSLLDTDAYKLHMQQAVFHHYRHI-TVAAEFRCRSDE---LLGVYADEIRHQVTLMGQLALTSDEFIYLSSLPFFQDDYLHWLRDFRFKPEQVSVAVHDGKL------DIRIAGLWCEVIMWEVPLLAVISEIVHRRRSTQVTTDQAVQQLRTKLEQFNALSADIDITHFKLMDFGTRRRFSREIQHTVVSTLKDEFPYLVGTSNYDLARTLALAPVGTQAHEWFQAHQQISPTLANSQRVALQVWLDEYPNQLGIALTDCITMDA-FLRDFD-LA----FANRYQGLRHDSGDPIEWGEKAIAHYEKLGIDPMKKVLVFSDNLDLEKALFLYRHFYQRIKLVFGIGTRLTCDIPDVKPLNIVIKLVECND----KPVAKLSDSPGKTICQDPAFVDQL----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3OS4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1736 -7968 -4.59 -23.50 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -4.59 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_3OS4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OS4-query.scw PDB file : Tito_Scwrl_3OS4.pdb: