Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRLFIAEDQRMLLGALGSLLDLEEDMTVIGQALNGEEALHAILKLEPDVCIMDIEMPVRSGLNVAEELMKKGCVSKVIILTTFARPGYFERAVKAGAHGYLLKDGEIDDLADAIRKCVKGKRVFSPELTFNMMRDENPLTVREQEILRLAALGKTTKDITLELYLSQGTVRNYISEIIQKLNAKNRTEAASIAEEKGWI 4E7O Chain:B ((21-148)) -MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEKESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVLKERSIADLMQTLHTVLEGRKEYSPEL-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4E7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 664 -96551 -145.41 -754.30 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -145.41 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_4E7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E7O-query.scw PDB file : Tito_Scwrl_4E7O.pdb: