Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLKVMTVFGTRPEAIKMAPLVLELKKY-PEIDSYVTVTAQHRQMLDQVLDAFHIKPDFDLNIMKERQTLAEITSNALVRLDELFKDIKPDIVLVHGDTTTTFAGSLAAFYHQIAVGHVEAGLRTGNKYSPFPEELNRQMTGAIADLHFAPTGQAKDNLLKENKKADSIFVTGN-TAIDALNTTVRDGYSHPVLDQVGEDKMILLTAHRRENLGEPMENMFKAIRRIVGEFEDVQVVYPVHLNPVVREAAHKHFGD-SDRVHLIEPLEVIDFHNFAAKSHFILTDSGGVQEEAPSLGKPVLVLRDTTERPEGVEAGTLKLAGTDEENIYQLAKQLLTDPDEYKKMSQASNPYGDGEASRRIVEELLFHYGYRKEQPDSFTGK 1V4V Chain:A ((4-371)) -GMKRVVLAFGTRPEATKMAPVYLALRGIPG-LKPLVLLTGQHREQLRQALSLFGIQEDRNLDVMQERQALPDLAARILPQAARALKEMGADYVLVHGDTLTTFAVAWAAFLEGIPVGHVEAGLRSGNLKEPFPEEANRRLTDVLTDLDFAPTPLAKANLLKEGKREEGILVTGQTGVDAVLLAAKLG-RL---PEGLPEGPYVTVTMHRRE-NWPLLSDLAQALKRVAEAFPHLTFVYPVHLNPVVREAVFPVL-KGVRNFVLLDPLEYGSMAALMRASLLLVTDSGGLQEEGAALGVPVVVLRNVTERPEGLKAGILKLAGTDPEGVYRVVKGLLENPEELSRMRKAKNPYGDGKAGLMVARGVAWRLGLGPRP-------

General information: TITO was launched using: RESULT: Template: 1V4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2061 -19026 -9.23 -52.12 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -9.23 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1V4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V4V-query.scw PDB file : Tito_Scwrl_1V4V.pdb: