Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKTASKFSVMFFML------LALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNLLPQSE--------------DS-QASGKPEF-VNV------TSHGKTILEAVSDVSIKDP-PVYSDHLKVILLGEKLMRNQNVDQV-LNHF-IRDDELRR-SSYLMAARGNAADVFTKGNPNQQ-QPMPSEK-LIDLTTHSGYNGKIMI--PLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVGFLSADETQTLS-------WVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPV----FHISV-K-TKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVM-DKLQHEY------KTDP----VFLSDHIRIQHPDYWNKVKGHWDEIFSE---------T-DFKYDISFKIIN-FGTVGK 4OO3 Chain:A ((27-465)) EGFTGAPGEVKLITLDPGHFHAALVQKVSYPQVSKDVYVYAPTGFDVDEHLKRIQGFNTRAENPTAWNEIVYTGDDYLEKMLAEKKGNVMIQAGNNGKKTEYIKKTLEAGINVLSDKPMAINSQSFKLLEECFAIAKQKNI---MLYDIMTERNEITTMLQRELSTIPAVYGEQLKGSPEEPAIVKESVHHLFKLVDNKPLTRPVWYLDVNQQGEGIVDVTTHLVDLVQWEAFPDQIIDYRKD-IELIDANRWTTSISPEEFKQVTGTDAYPDFLKKDVENDTLKVYCNG-DIVYKIKGVTAKVSVIWNYTFPKGGGDTHFSVMKGSKADLVIRQGKEQNYQPELFVEAVKGVDLAAYEKDLTA-SMEKVSAEYPGVALNKVGDGVWQVEIPAKYRVGHEAHFGQVTEHFLDYLKEGKLPDWEVPNMLAKYYTTTSALDMAKAKT-

General information: TITO was launched using: RESULT: Template: 4OO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1821 66839 36.70 182.12 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 36.70 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.139

(partial model without unconserved sides chains): PDB file : Tito_4OO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OO3-query.scw PDB file : Tito_Scwrl_4OO3.pdb: