Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLHKERRIGRLSVLLLLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI 1WRQ Chain:B ((1-147)) -TLHKERRIGRLSVLLLLNE----TQVEELERDGWKVCLGKVGSMDAHKVIAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI

General information: TITO was launched using: RESULT: Template: 1WRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 695 -98183 -141.27 -686.59 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.99 3D Compatibility (PKB) : -141.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_1WRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WRQ-query.scw PDB file : Tito_Scwrl_1WRQ.pdb: