Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIAFLNSLVV----TSPGFYKAEKITLDEVKQWLKH--YDGRYKSFIGHKSTAQFLQKLLGIRIEQNRKTFRHMKYQKAICFSLYERYPENVLLTQRDLEKARYQFYLLTRLD 2J85 Chain:A ((2-104)) GKVFLTNAFSINMLKEFPTTITIDKLDEEDFCLKLELRLEDGTLINAIGHDSTINLVNTLCGTQLQKNRVEVKMNEGDEALIIMISQRLEEGKVLSDKEIKDM-----------

General information: TITO was launched using: RESULT: Template: 2J85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 317 -47983 -151.37 -494.67 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -151.37 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2J85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J85-query.scw PDB file : Tito_Scwrl_2J85.pdb: