Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLDKDSVKAIDVQTASLQSYISSPEKQKSLYKRTLFVVSISQIFGGAGLAAGVTVGALIAQQ-MLGTDAFAGLPSALFTLGSAGSALIVGRLSQRYGRRTGLSAGFMIGGLGAIGVIMAAIINS-IFLLFISLLIYGAGTATN----LQARYA-GTDLANHKQRATAVSITMVFTTFGAVAGPSLVNVMGDFALSIGVPSLAGPFILAAAAYMLAGVVLFIMLRPDPLVIARTIEAANEEPGDKGHLAATEH-TENKKGIIVGATVMVLTQIVMVAIMTMTPVHMR----HHGHDLGAVGLVIGFHIGAMYLPSLVTGVLVDRLGRTAMAISSGTTLLLAGVIAAFAPGESMILLVIALSLLGLGWNFGLISGTALIVDSTDTATRAKTQGTV-DVLIALSGAAGGALSGMIVAGSSYLALSLIGGILSLLLIPVVVWSRGR 2CFQ Chain:A ((7-392)) ---------------------------------TNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVS--RRSNFEFGRARMFGCVGWALGASIVGIMFT----INNQ---FVFWLGSGCALILAVLLFFAKTDAPSSA-TVANAVG-A-NHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFA--TSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF----------

General information: TITO was launched using: RESULT: Template: 2CFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1750 -274929 -157.10 -737.07 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -157.10 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_2CFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFQ-query.scw PDB file : Tito_Scwrl_2CFQ.pdb: