Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDEFTIGQVFKTK-SLKVSKDDIMRFAGE-FDPQYMHVDEEKASKGRFNGIIASGIQTLAISFKLWIEEGFYG------DDIIAGTEMNHMTFIKPVYPDDELFTIVEVLDKQPKRNELGILTVLLSTYNQKEVKVFEGELSVLIKR 4RLJ Chain:A ((3-145)) --LSADIVGMHYRYPDHYEVEREKIREYAVAVQNDDAWYFEEDGAAELGYKGLLAPLTFICVFGYKAQAA--FFKHANIATAEAQIVQVDQVLKFEKPIVAGDKLYCDVYVDSVRE-AHGTQIIVTKNIVTNEEGDLVQETYTTLAGR-

General information: TITO was launched using: RESULT: Template: 4RLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -72882 -120.67 -539.87 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -120.67 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_4RLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RLJ-query.scw PDB file : Tito_Scwrl_4RLJ.pdb: