Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGTEKVTALKAKHQSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVG------GIQPAVIATLFLVVRVLDAIFDPIMGLILDKTNTRW-GKARPYLLWVAFPFALFTFLCFTTP-HFGETGNMVYAYVTYILLGMSFSMQTIPVNSLTGRMTNSVEE---RTVLTTTRMILVYIGILLSISCATPLATAIGGEDQAFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKK--RKKQGI------------------------------------------------------------------------K-KTLSV-LFKNKPLLMLISSFLAFAIGFNIKLSTMVYYFTYNVNHKE-FVFMGTVLFFGAALISNLFIPF--------FSEKWGRKQVMIITAALSLIS-YAGLHF------TP-YSSIPLIFIWLF-ASGFFTTPLNTLAWGMVADCVDYAEWKTGIRADGVVISSMSFINKLGVALAGSFSAIYLGIAGYVANTDQTVASLNAIKNMNALIPGFFILLSIILIAFYPLTEKRYKHIISELEQRSAR 4UVM Chain:A ((17-468)) -----------------------------IIASEACERFSFYGMRNILTPFLMTALLLSIPEELRGAVAKDVFHSFVIGVYFFPLLGGWIADR----FFGKYNT-ILWLSLIYCVGHAFLAIFEHS------VQGFYTGLFLIALGSGGIKPLVSSFMGDQFDQ-SNKSLAQKAFDMFYFTINFGSFFASLSMPLLLK-------NFGAAVAFGIPGVLMFVATVFFWLGRKRYIHMPPEPKDPHGFLPVIRSALLTKVEGKGNIGLVLALIGGVSAAYALVNIPTLGIVAGLCCAMVLVMGFVGAGASLQLERARKSHPDAA-VDGVRSVLRILVLFALVTPFWSLFDQKASTWILQANDMVKPQWFEPAMMQALNPLLVMLLIPFNNFVLYPAIERMGVKLTALRKMGAGIAITGLSWIVVGTIQLMMDGGSALSI-F-WQILPYALLTFGEVLVSATGLEFAYSQAPK-------AMKGTIMSFWTLSVTVGNLWVLLANVSVKSPT----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1534 -249463 -162.62 -718.91 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -162.62 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_4UVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UVM-query.scw PDB file : Tito_Scwrl_4UVM.pdb: