Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKHISMLFVFLMAVMVLSACNSSESSSNSEVSSSKTRTVKHAMGTSDNIPANPKRIVVLTNEGTEALLALGIKPVGAVKSWKGDPWYDYLKDDMKGVKNVGLETEPNVEAIAELKPDLIIGNKVRQEKIYDQLNAIAPTVF-----AESLAGNWKDNLTLYANAVNKADKGKEVIADFDKRVSDLKNKLGDQTNKTVSVVRFLS-GESRIYYTDSFPGIILDQLGFKRPEKQVELFKKQKDQFTFSTDSKESIPDMDADVLFYFTYKADNAKENEKWANQWTSSSLWKNLKAVKSGNAHEVDDVVWTTAGGIKAANYLLDDIETYFLKTK 1EFD Chain:N ((2-263)) --------------------------------------------------GIDPNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSE-PPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSA-GYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQ------GETNFWGSTAVSIDRLAAYKDVDVLCFD------HDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVW--FYGATLSAMHFVRVLDNAIG---

General information: TITO was launched using: RESULT: Template: 1EFD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 1334 -148578 -111.38 -580.38 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain N : 0.69 3D Compatibility (PKB) : -111.38 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1EFD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFD-query.scw PDB file : Tito_Scwrl_1EFD.pdb: