Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQSQPVLTIVVPCFNEEEVFQETSHQLTEVVDDLIEEKLIAEDSKILFVDDGSKDRTWALIAMESIRNK--KVTGLKLACNVGHQKALLAGLHKAKNRSDCVISIDADLQ-DDISVIRDFMLKYHE--GCEIVYGVRRSRKTDT---FFKRTTALGFYRLMNKL-G--IKLIYNHADFRLMNKRSLEELERYPEANLF---LRGIVPMIGF--KSAEVLYDRKERFAGKTKYPLKKMLSFAFNGITSFSVAPIRFFTLLGFVLFFLSAVAGIGAFIQKLLGHTNAGWASLIISIWFLGGLQLMGIGIIGEYIGTIFSEVKRRPKYAIDIDLYNEQLSPLQRQEKERLEKKYS 3F1Y Chain:A ((91-309)) --ELGLTVSAVLPSRNVADTVGGIIDEIHALNERA------PLIDQILVVDADSEDGTAGVAASHGAEVYSENELMSGYGDAHGKGDAMWRALSVTR--GDLVLYIDADTRDFRPQLAYGVLGPVLEVPGVRFVKAAYRRP----EEDGGGRVTELTAKPLFNLFYPELAGFVQPLAGEFVADRELFCSI-PFLTGYAVETGIMIDVLKKVGLGAMAQVDLGERQNR------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3F1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1013 -120469 -118.92 -614.64 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -118.92 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_3F1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F1Y-query.scw PDB file : Tito_Scwrl_3F1Y.pdb: