Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNWMEFISSIVKSIIWPVAIIILVFKLKQPVSNLISTLAKIKYKDWEFEFTIDQKLDKISFQLNKENQYGTD-DQNLKDSLNLESNSEITEETHSHYNEKTSIKGDNDLLLKMEMIRLYGAVKNLYEKVSEKDDAADKSQGIGMLYMVDYLKRHKILNDSFAESLLDIMNIVHDLEKSHISQSYIMNKFFWNVDKSVKRLMEITSNYVAENTAGETSN 5DFN Chain:A ((18-206)) TLKYKELIAGELMRITHK----LLIQKLQQQ--EVIIKIQEIA-KDQQLYDTLS-----IQYQVDQKEQYYAKIAQSLEDFVSI---SALKMVSYIYPNISYQVS-----------IGFFQNILDIATKTVKDRGALGCN--------------YKYLKDKLTKAL-NLQQISYPL----ISESYISYLVHLFQDFNIIEIENEHKFYYKQ--------

General information: TITO was launched using: RESULT: Template: 5DFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 605 15203 25.13 92.70 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 25.13 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_5DFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFN-query.scw PDB file : Tito_Scwrl_5DFN.pdb: