Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDIEVMKAIDKELPQYTFEFKTMDFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNYSPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKYNKTHGSPIHLKYAGQGSNDTANQIETGRADATIATPFAVDFQ-NKTHA-FRQKTVGD-----VLLDTEVYFMFNKGSQTLADDTDQAIKKLEKNGTLKKLSRKWLGADYSKSSFEK 1LST Chain:A ((3-234)) -------------------------------PQTVRIGTDTTYAPFSSKDAKGEFIGFDIDLGNEMCKRM-QVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQEIAFSD-KLYAADSRLIAAKGSPIQPTLESLKGKHVGVLQGSTQEAYANDNWRT-K-GVDVVAYA-NQDLIYSDLTAGRLDAALQDEVAASEGFLKQPAGKEYAFAGPSVKDKKYFGDGTGVGLRKDDTELKAAFDKALTELRQDGTYDKMAKKYFDFN--------

General information: TITO was launched using: RESULT: Template: 1LST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1220 -40593 -33.27 -180.41 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -33.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_1LST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LST-query.scw PDB file : Tito_Scwrl_1LST.pdb: