Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVFVARQPIFNR-KEQVVAYELLYRES-EENV-Y-SA---KD------GDQATTDLVINSFLNIGIEKL--TEGKRCFVNFTESLMFSNLP----------TSFNPKQLVIEILEDI--PITPALISRCKELKKMGYMLALDDFYAINPQDEDLLEKLMSY-IDILKIDFLKTTRM--------ERRTILQTYGCRGLIFLAEKVETRKEYKQAVQDGFQLFQGYFFSEPRIISGHDLSTHFYSYYELLNELSKEQPNIKRVTEYIERDLSLSYQILKFLNSSHSRLSQKIESIQQAIMLLGFNEIKRWIYILSFKDLSRKGHSSKHEIIKISLIRAKLCELLARKTSRPQPASYMLIGMFSLIDTLLHREIEEIVQELPLKDEVGQALLGHQNDYYQMLELVKLIESNNWDTCSELGNQLDKEEAYECYLEALEWCHNLMDAK 4RNI Chain:A ((19-247)) -EFVLHYQPQFTGDGRRLTGAEALLRWQHPRRGLVPPSEFIPVLEEIGLVAQVGDWLLAEACKQLRSWHKAKVRVPKVSVNLSARQFADGQLGERIAAILYETGIPPACLELELTESILMSDVAEAMQILSGLKRLGLAIAVDDFGT-GYSS---LNYLKQFPIDVLKIDRSFVDGLPHGEQDAQIARAIIAMAHSLNLMVIAEGVESQAQLDFLREHGCDEVQGYLFGRPMPA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RNI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 761 -17002 -22.34 -88.09 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -22.34 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_4RNI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNI-query.scw PDB file : Tito_Scwrl_4RNI.pdb: