Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESD-KWSFEAETETVAGIPAGTDNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRVPN-ADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV 3HUL Chain:A ((2-292)) ------SLRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIG--GGIPHDETNVIIETALNLAPN----LTPHHLVMTCDIPPARGLGSSSAAVVAGIELANTLAELNLSKEEKVRIAAEIEGHPDNVAPAVLGNWVVGAKLDGE-DFYVRHLFP-CALIAFIPKAELL--------PDTLPFKEAVQASSIANVMIAAILRNDMTLAGEMMERDLW-----SQLVPHLAQIRDVAKNQGAYAACLSGAGPTVLVFAPRNLANKLQTSLQTLEIDADVLLLDVEGSGAEVFREG-------

General information: TITO was launched using: RESULT: Template: 3HUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1614 -69716 -43.19 -253.51 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -43.19 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3HUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HUL-query.scw PDB file : Tito_Scwrl_3HUL.pdb: