Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIPYLQEQGFSKTELGLVLAAVSIAYGFSKFIMGMVSDRCNPRYFLATGLFLSAIVNILFVSMPWVTSSVTIMFIFMFINGWFQGMGWPPCGRTMAHWFSISERGTKMSIWNVAHNIGGGILAPLVTLGIAMFVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDY--PKHAFKNQEKELTTKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFSPEDSRWSYFLYEYAGIPGTILCGWISDRFFKSRRAPAGVLFMAGVFIAVLVYWLNPAGNPLVDNIALISIGFLIYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFANAIMGFVVDRFNWNGGFIMLISSCILAIVFLALTWNTGKRAEHV 4IU9 Chain:A ((12-454)) LLRDWKPEN--PAFWENKGKHIARRNLWISVSCLLLAFCVWMLFS-AVTVNLNKI-GFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVI---------------------------FVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIALWLLSLLYLATFGSFIGFSAGFAMLAKTQ--FPDVNILRLAFFGPFIGAIARSVGGAISDKF-GGVRVTLINFIFMAIFSALLFLTLPGTGSG---NFIAFYAVFMGLFLTAGLGSGSTFQMIA----VILGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVY---------

General information: TITO was launched using: RESULT: Template: 4IU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2153 -326357 -151.58 -828.32 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -151.58 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_4IU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IU9-query.scw PDB file : Tito_Scwrl_4IU9.pdb: