Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRIRKAPAGILGFPVAPFNTQGKLEEEALFQNIEFLLNEGLEAIFIACGSGEFQSLSQKEYEQMVEVAVSAAGGKVPVYTGVGGNLSTALDWAQLSEKKGADGYLILPPYLVHGEQEGLYQYAKTIIESTDLNAILYQRDNAVLSVEQIKRLT-ECEQLVGVKDGVGNMDLNINLVYTIGDRLGWLNGMPMAEVTMPAYLPIGFHSYSSAISNYIPHISRMFYDALKNGNDELVKELYRHVILPINDIRKQRKGYAVSLIKAGMEIMGLNVRNTARPPVGPVEKDHYQQLEAILKQAADRFPKKAATV 5HWJ Chain:B ((12-296)) --------SGLLSFPVTHFDAEGRFAADSYREHVEWLAGYKAPVLFAAGGTGEFFSLKPDEIPTIVAAAKEVAG-ETAIVSGCGYGTEIAVDIARSVEKVGADGILLLPHYLIDAPQEGLYAHIKKVCQSVGIGVMVYNRDNSVLQADTLARLCDECPNLVGFKDGTGDIGLVRQITAKMGDRLMYLGGMPTAELFAEAYLGAGFTTYSSAVFNFVPGLANEFYAALRAGERATCERILVDFFYPFMAIRNRAKGYAVSAVKAGVRLQGFNA-GPVRAPLKDLTNEEIGMLEALI--------------

General information: TITO was launched using: RESULT: Template: 5HWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1595 -215067 -134.84 -757.28 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -134.84 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_5HWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HWJ-query.scw PDB file : Tito_Scwrl_5HWJ.pdb: