Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVRYQIEQLGDSAMMIRFGEE-INEQVNGIVHAAAAYIEEQPFPGFIECIPAFTSLTVFYDMYEVYKHLPQGISSPFESVKRDVEERLAEIAEDYEVNRRIVEIPVCYGGEFGP-------------------DLEEVAKINQLSPEEVIDIHTNGEYVVYMLGFA-PGFPFLGGMSKRIAA--PRKSSPRPSIPAGSVGIAGLQTGVYPIS-TPGGWQLIGKTPLALFRPQE------NPPTLLRAGDIVKFVRISEKDYHAYKEESN 3VA7 Chain:A ((836-1101)) --PKVTYRQAGDRYILVEYGELEFDLNICYRINRLIHQVERHQTVGIVEMSQGVRSVLIEFDGSKINQKALLK------CLIAYESEIQFDK--NWNVKSKIFKLPMAFEDSKTLDCVTRYRETIRSEAPWLPNNVDFIADVNDIDRNDVKNMLYSAKFMVLGLGDVFLGSPCAVPLDPRHRYLGTKYNPSRTYTARGVVGIGGMYMCIYNAEGSPGGYQLVGRTIT-AWDKLVIGDHPIDHPWLLTPFDQVEFYPVTEEELEVIIEDN-

General information: TITO was launched using: RESULT: Template: 3VA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1125 -142002 -126.22 -622.81 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -126.22 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3VA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VA7-query.scw PDB file : Tito_Scwrl_3VA7.pdb: