Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQKDMYVLGIETSCDETAAAIVKNGKEIISNVVASQIESHKRFGGVVPEIASRHHVEQITLVIEEAFRKAGMTYSDIDAIAVTEGPGLVGALLIGVNAAKALSFAYNIPLVGVHHIAGHIYANRLVEDIVFPALALVVSGGHTELV-YMKEHGSFEVIGETLDDAAGEAYDKVARTMGLPYPGGPQIDKLAEKGNDNIPLPRAWLEEGSYNFSFSGLKSAVINTLHNASQKGQEIAPEDLSASFQNSVIDVLVTKTARAAKEYDVKQVLLAGGVAANRGLRAALEKEFAQHEGITLVIPPLALCTDNAAMIAAAGTIAFEKGIRGAYDMNGQPGLELTSYQSLTR 3EN9 Chain:B ((6-322)) ------MICLGLEGTAEKTGVGIVTSDGEVLFN---KTIMYKP---GINPREAADHHAETFPKLIKEAFEV--VDKNEIDLIAFSQGPGLGPSLRVTATVARTLSLTLKKPIIGVNHCIAHIEIGKLTTEAEDP-LTLYVSGGNTQVIAYVSK--KYRVFGETLDIAVGNCLDQFARYVNLPHPGGPYIEELARKGKKLVDLPYT---VKGMDIAFSGLLTAAM----RAYDAGERL--EDICYSLQEYAFSMLTEITERALAHTNKGEVMLVGGVAANNRLREML-KAMCEGQNVDFYVPPKEFCGDNGAMIAWLGLLMHKNG-----------------------

General information: TITO was launched using: RESULT: Template: 3EN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1760 -213750 -121.45 -722.13 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -121.45 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3EN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EN9-query.scw PDB file : Tito_Scwrl_3EN9.pdb: