Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDVTEWKHVFKLDPNKDLPDEQLEILCESGTDAVIIGGSDGVTEDNVLRMMSKVRRFLVPCVLEVSAIEAIVPGFDLYFIPSVLNSKNADWIVG-------MHQKAMKEYGELMSMEEIVAEGYCIANPDCKAAALTEADADLNMDDIVAYARVSE-LLQLPIFYLEYSGVLGDIEAVKKTKAVLETSTLFYGGGIKDAETAKQYAEHADVIVVGNAVYEDFDRALKTVAAVKGE 2F6X Chain:B ((8-223)) -----KWRHITKLDPDRTNTDEIIKAVADSGTDAVMISGTQNVTYEKARTLIEKVSQYGLPIVVEPSDPSNVVYDVDYLFVPTVLNSADGDWITGKHAQWVRMHYENLQKFTEIIESEFIQIEGYIVLNPDSAVARVTKALCNIDKELAASYALVGEKLFNLPIIYIEYSGTYGNPELVAEVKKVLDKARLFYGGGIDSREKAREMLRYADTIIVGNVIYE---------------

General information: TITO was launched using: RESULT: Template: 2F6X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1050 -140111 -133.44 -673.61 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -133.44 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_2F6X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F6X-query.scw PDB file : Tito_Scwrl_2F6X.pdb: