Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLMNEFEKACETLRKFMAYMLEK-DMKSWTELWDENAVFEFPYAPEGSPKRIEGKAAIYDYIKDYPKQIHLSSFTAPTVYRSADSNTVIAEFQCDGHVIETGLPYRQSYISVIETRDGRIVRYRDYWNPLVVKEAFGGSFLQTEESGK 4J9A Chain:G ((2-123)) ---NAKEIVVHALR-----LLENGDARGWSDLFHPEGVLEYPYPPPGYKTRFEGRETIWAHMRLFPEYMT------VQFYETADPDLAIGEFHGD------------DYIAVWRTRDGQILLYRLFFNPLR-----------------

General information: TITO was launched using: RESULT: Template: 4J9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 419 -41157 -98.23 -395.74 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain G : 0.72 3D Compatibility (PKB) : -98.23 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_4J9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J9A-query.scw PDB file : Tito_Scwrl_4J9A.pdb: