Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIKHADSFGIELPAGAISVDWSKMQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKLLIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDSKDALSLSE-IPSSLVIVGGGVIGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQTAKTAIWK----SGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKG-IPVNGHMQTNVPHIYACGDAIGGIQLAHAAFHEGIIAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQARSIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEIVGVSMIGPDVTELIGQAA-AIMNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA 2EQ9 Chain:G ((9-450)) --LIVIGTGPGGYHAAIRAAQLGLKVLAVEAGEVGGVCLNVGCIPTKALLHAAETLHHLKVAEGFGLKAKP---ELDLKKLGGWRDQVVKKLTGGVGTLLKGNGVELLRGFARLVGPKEV----EVGGERYGAKSLILATGSEPLELKGFPF-GEDVWDSTRALKVEEGLPKRLLVIGGGAVGLELGQVYRRLGAEVTLIEYMPEILPQGDPETAALLRRALEKEGIRVRTKTKAVGYEKKKDGLHVRLEPAEGGEGEEVVVDKVLVAVGRKPRTEGLGLEKAGVKVDERGFIRVNARMETSVPGVYAIGDAARPPLLAHKAMREGLIAAENAAGKDSAFDYQ-VPSVVYTSPEWAGVGLTEEEAKRAGYKVKVGKFPLAASGRALTLGGAEGMVKVVGDEETDLLLGVFIVGPQAGELIAEAALALEMGATLTDLA-LTVHPHPTLSESLMEA-----------

General information: TITO was launched using: RESULT: Template: 2EQ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 2583 -256939 -99.47 -590.66 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain G : 0.81 3D Compatibility (PKB) : -99.47 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_2EQ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQ9-query.scw PDB file : Tito_Scwrl_2EQ9.pdb: