Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFSEECRSAAAEWWEGSFVHPFVQGIGDGTLPIDRFKYYVLQDSYYLTHFAKVQSFGAAYAKDLYTTGRMASHAQGTYEAEMALHREFAELLEISEEERKAFKPSPTAYSYTSHMYRSVLSGNFAEILAALLPCYWLYYEVGEK---LLHCDPGHPIYQKWIGTYGGDWFRQQVEEQINRFDELAENSTEEVRAKMKENFVISSYYEYQFWGMAYRKEGWSDSAIKEVEECGASRHNG 2GM7 Chain:C ((14-218)) -----ELRRRADGIWQRILAHPFVAELYAGTLPMEKFKYYLLQDYNYLVNFAKALSLAASRAPSVDLMKTALELAYGTVTGEMANYEALLKEVGLSLRDAAEAEPNRVNVSYMAYLKSTCALEGFYQCMAALLPCFWSYAEIAERHGGKLRENPVH-VYKKWASVYLSPEYRGLVERLRAVLDS-SGLSAEELWPYFKE----ASLYELEFWQAAY-----------------------

General information: TITO was launched using: RESULT: Template: 2GM7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 876 -107017 -122.17 -529.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -122.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_2GM7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM7-query.scw PDB file : Tito_Scwrl_2GM7.pdb: