Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKAVILDEQAIRRALTRIAHEMIERNKGMNNCILVGIKTRGIYLAKRLAERIEQIEGNPVTVGEIDITLYRDDLSKKTSNDEPLVKGADIPVDITDQKVILVDDVLYTGRTVRAGMDALVDVGRPSSIQLAVLVDRGHRELPIRADYIGKNIPTSKSEKVMVQLDEVDQNDLVAIYENE 4P83 Chain:D ((5-181)) ---KAVIMDEQAIRRALTRIAHEIIERNKGIDGCVLVGIKTRGIYLARRLAERIEQIEGAPVPVGEIDITLYRD------DDDEPLVKGTNVPFPVTERNVILVDDVLFTGRTVRAGMDAVMDLGRPARIQLAVLVDRGHRELPIRADFVGKNVPTSRSELIVVELDEVDQNDQVSIHEN-

General information: TITO was launched using: RESULT: Template: 4P83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 892 -128583 -144.15 -751.94 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain D : 0.95 3D Compatibility (PKB) : -144.15 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.641

(partial model without unconserved sides chains): PDB file : Tito_4P83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P83-query.scw PDB file : Tito_Scwrl_4P83.pdb: