Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MNKHKKIPLRKCVVTGEM---------KPKKELIRVV-----RSKEGEISVDPTGKKNG-RGAYLTLDKECI-----LAAKKKNTLQNQFQSQIDDQIFDELLELAEKVKK 5EK5 Chain:A ((6-123)) QRYISIRNTDTIWLPGNICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLETECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITV

General information: TITO was launched using: RESULT: Template: 5EK5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 316 -7995 -25.30 -87.86 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -25.30 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5EK5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EK5-query.scw PDB file : Tito_Scwrl_5EK5.pdb: