Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEA--SANRLAIHINVDTGMGRLGV----RTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIKQLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV 4Y2W Chain:B ((7-378)) -----RPTRVEVYLDAITHNFREIKKIVGKNVKIMAVIKGDAYGHGASYVAKFLEKEGVDYFGVATTEEALELREKGIKTPILIFGYTPPTQLRQIVKHDLTQTVYDIKYAKE----LEKESLKQNKRAKVHIKIDTGLGRIGYIDFDLAQKEILEMANM----RGLILEGIYSHFAAASEDDRDYCKEQFDKFMNLISSLEKKRLKIPLKHIANAAAILNLNYSHLDMVRPGIILFGAYPSKRVERK--VELRETLRFTTRVVHLKDV-PAGFFIGYGKSFVTKRKSVIATIPVGYADGLDRRLSNNYKLLLKGKYVPIVGRVCMDQCMIDVTDVEGVEIGDEVVIIGTQNNETVSVESMADKIETIPQEVFSRISRRVPRVYFYDG------------

General information: TITO was launched using: RESULT: Template: 4Y2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2063 -71842 -34.82 -196.29 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -34.82 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4Y2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y2W-query.scw PDB file : Tito_Scwrl_4Y2W.pdb: