Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW 3VZL Chain:B ((1-185)) ----------------------------ASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGHFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFTNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW

General information: TITO was launched using: RESULT: Template: 3VZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1084 -34858 -32.16 -188.42 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -32.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.904

(partial model without unconserved sides chains): PDB file : Tito_3VZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VZL-query.scw PDB file : Tito_Scwrl_3VZL.pdb: