Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNRLWKVRPLSKMWGIGGRMERNLNRMGISTVGQLAKFPLELLEKKFGI-MGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILFRDYTKREEIKAVLLEICEEVARRARTYNKVGRTISLGIGYSKDELGGGFHRSKTIDFPTNITMDIYKCCLMFFNKFYSGKKQ 4F50 Chain:A ((125-325)) LARKIKQEILEKEKITVTVGVAPNKILAKIIADKSKPN---GLGVIRPTEVQDFL-NELDIDEIPGIGSVLARRLNELGIQKLRDILSKNYNELEKITGKAKALYLLKLAQNKYSEPVENK---SKIPHGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKR--IPKAIHVVAVTEDLD---IVSRGRTFPHGISK-ETAYSESVKLLQKILE----

General information: TITO was launched using: RESULT: Template: 4F50.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 665 -50421 -75.82 -252.11 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -75.82 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4F50.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F50-query.scw PDB file : Tito_Scwrl_4F50.pdb: