Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------MIHKNWLEKETIKKVKCVQTNAKKYIVNRVLTPGKEYEVKNETEEFLFVVDNTNKVGGYYKEYFEEM------------------------------------------------------------------------------------------------------------------------ 4QTS Chain:A ((1-369)) MKMVVLKPKINSKFHFKIFHSNSLFSAIVNNYIKLYGREDLEKNIEKIKNIRLSSLLYKIKNIYLIPKPEHPEFYPKDIKKIQFFSIKAYKELLDNELDWKNKIKHIVDYQTINKSIVISEKEIEEIKRIFGIKAEKLKHAKISLISKHLEQKVADKGQLYNIEFIKLNENVEFYFLIDYNNE----DKEFIKKLEASIKLIEDEGLGGAGFFEKVEIVDLPEDFNEILDENSKYNNLEYKMLLGVGIPNKDDIKNIEYYKLIEIGGYIYSLECLTKPKRNILALTEGSIVKNDFIGDVKDVYTHGKPILLPFNP

General information: TITO was launched using: RESULT: Template: 4QTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 99 15542 156.99 246.70 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : 156.99 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.094

(partial model without unconserved sides chains): PDB file : Tito_4QTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QTS-query.scw PDB file : Tito_Scwrl_4QTS.pdb: