Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSYPESLKKETEEIKERVRNGNI---KEDKI---KEIAETTVEFLKSEEKRHKYFSEVAAAMADNLSEFFKSYLKGE 3ZIE Chain:A ((44-86)) ----VTGL-NEVPEIKREIYDGNIVVADIAFIKHDKLTLDRVLKDLRQ------------------------------

General information: TITO was launched using: RESULT: Template: 3ZIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 69 7177 104.01 193.96 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 104.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.653

(partial model without unconserved sides chains): PDB file : Tito_3ZIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZIE-query.scw PDB file : Tito_Scwrl_3ZIE.pdb: