Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 3Q8V Chain:B ((1-148)) -MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMELAETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIRGDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLY

General information: TITO was launched using: RESULT: Template: 3Q8V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 683 -72891 -106.72 -492.51 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.93 3D Compatibility (PKB) : -106.72 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_3Q8V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q8V-query.scw PDB file : Tito_Scwrl_3Q8V.pdb: