Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDFLSKTPEP-PYYAVIFSSVKSENDTGYGETAERMVSLAADQPGFLGVESVR---EADGRGITVS-YWDSMDAINHWRHHTEHQAAKEKGRSVWYESYAVRVAKVDRQRLFQENTND 3BDE Chain:A ((18-116)) ---------QGMIRHTVVFTLKHASHSLEEKRFLVDAKKILSAIRGVTHFEQLRQISPKIDYHFGFSMEFADQAAYTRYNDHPDHVAFVRD----RWVPEVEKFLEIDYVPL-------

General information: TITO was launched using: RESULT: Template: 3BDE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 341 -5252 -15.40 -55.87 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -15.40 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_3BDE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BDE-query.scw PDB file : Tito_Scwrl_3BDE.pdb: