Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLERLNQDMKLYMKNREKDKLTVVRMVKASLQNEAIKLKKDSLTEDEELTVLSRELKQRKDSLQEFSNANRLDLVDKVQKELDILEVYLPEQLSEEELRTIVNETIAEVGASSKADMGKVMGAIMPKVKGKADGSLINKLVSSQLS 2AO9 Chain:A ((13-132)) -----------MMAKLDELKQKLTAKQIQAAYLLVENELMEEEKRTQDE----MANELGINRTTLWEWRTKN---------QDFIAFKSEVADSFLAEKREQVYSKLMQLILGPQPS--VKAMQLYMQRFGLLTDKKVIEGDL-----

General information: TITO was launched using: RESULT: Template: 2AO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 273 -4062 -14.88 -34.71 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -14.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_2AO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AO9-query.scw PDB file : Tito_Scwrl_2AO9.pdb: