Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIMGTALA---RKDLGR---HIITTKIEHHAVLHTCEKLEGDGF-DITYLDVDQ-NGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKE----------HKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPL---LFGGEQERKRRAGTENVPGIVGLKEAIKLSSEE--------RDEKNEKYQSFKAIFADTL----RDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVSSGSACTAG-SVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT 3A9Z Chain:B ((19-428)) --KVYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQ-----TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGV---------ERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSC-GIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQL-

General information: TITO was launched using: RESULT: Template: 3A9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1995 77482 38.84 215.23 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 38.84 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3A9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9Z-query.scw PDB file : Tito_Scwrl_3A9Z.pdb: