Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVELLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICTEEL-EHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVYSKEIGSAAAPTAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFR---PVSADEVEEHN--MHAEFYQMSEETAAALNKVR--ENGGRIISVGTTST--RTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGRENILRAYNHAVEEEYRFFSFGDAMLII- 4MFL Chain:A ((23-345)) MD--------PETVRIALGLEERTAAWLTELDEL------GPPAEPVRLPRG------EEARDLLRRL----EVPELDAEEIVAAAPDPDRDPALWWLLERTHHAIVRHMGDHRAKPRGGPPLPYEGGAAARYFHVYVFLATVPAVRRFHAERGIPDEVGWETLTQLGELVAIHRRKYGQGGMNMQWWTTYHLR--GILYRLGRLQFSLATGKDGTPHLGLAVPEWGGPLLPKAYDESLHRARPFFDRHFPEHGAR-VAWGSSWMLDPQLEEYLTEDSNIIQLARFWTLTDSAPEPGNADGDSSILEFVFRYNG--QPLDELPQRSSLERAVIAHLKAG-RHWHMRTGFVKLP

General information: TITO was launched using: RESULT: Template: 4MFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1416 -30042 -21.22 -96.29 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -21.22 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_4MFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MFL-query.scw PDB file : Tito_Scwrl_4MFL.pdb: