Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYP--GGAVLPIYDKLYNSGLVHILP-RHEQGAIHAAEGYARVSGKP--GVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVAT------SVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIATTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKL---VEAVSSAKKPVILAGAGVLHGKASEELKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTG-NLKHFARNAKIAHIDIDPAEIGKIMKTQIPVVGD--SKIVLQELIKQDG--KQSDSSEWKKQLAEWKEEYPLWYVDNEEE-GFKPQKLIEYIHQFTKGEAI-VATDVGQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILNNACLGMVRQWQEIFYEERYSESK---FASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMVAPGKGLHEMVGVKP 5AHK Chain:B ((35-581)) ---------------------------------NVLYGFELIGGMITHLVDSINLLGKTKLVSMHHEQGAAFAASAVSRVTHHKTLGLALATSGPGATNLITGIADCWLDSHPCIFLTGQVNTHELKGKRDIRQQGFQELDSVALVTSITKYAYQIKSADELVPCLRKAIQIAKEGRPGPVLLDIPMDIQRADIDEALLN---------------IAMSDLDFIINKLQNAKKPLLLIGGGAVNSSGFQKWLEQIELRGIPYVASLKGAEKIKASD-LYLGMLGAYGTRAANHAVQNCDLLLVLGSRMDVRQTGAQPEDFARNAEIIQIDLQEGQLNN------RVIADFSYQIELSEYFSRFSPLQIPVNNDWSVWTALLKEKFRVTFIDEYTTWNLSPFGLFTQLNKLTERVALDYILDVGNNQMWAAHTLRLNAQQAMHHSGGLGSMGFAIPAAIGACYAGKKPIIV-ITGDGGAQLNIQELDIIARDKLPILTIVMNNHSLGMVRGF---------SSTYWNGYTSQ--FKKIGEAYRVESKTIISMQAFSSALESFLESPRPLLLEVSMSDARECRPRLEYGRAIDQ------

General information: TITO was launched using: RESULT: Template: 5AHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3059 13699 4.48 27.34 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 4.48 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_5AHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AHK-query.scw PDB file : Tito_Scwrl_5AHK.pdb: