Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQQNQKISNPQTPVPTTSEMNDRDFVNELLTTEKYMTTAYCTALHEFSHESLYQDIQSIFDESQKAQRKLYDLMFQYGWYSVEAEDSQKLQQSYQKFQQTIQQQSPYQQ 1WIL Chain:A ((1-89)) ---GSSGSSGPREPVVNDEMCD----VCEVWTAESLFPCRVCTRVF---HDGCLRRMGYIQGDSAAEVTEMAHT--ETGWSCHYCDNINLLLT---------EESGPSSG

General information: TITO was launched using: RESULT: Template: 1WIL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 205 -19521 -95.22 -219.34 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -95.22 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_1WIL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WIL-query.scw PDB file : Tito_Scwrl_1WIL.pdb: