Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTENAKTNQTLVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYLG 1N1Q Chain:D ((6-148)) ---------QQLVAVLLNRQVANWVVLYVKLHNFHWNVNGPNFFTLHEKFEELYTEASGHIDTLAERVLSIGGSPIATLAASLEEASIKEATGGESAAEMVSSVVNDFVDLVGELKVARDVADEADDEATADMLDAIEAGLEKHVWMLEAFL-

General information: TITO was launched using: RESULT: Template: 1N1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 602 -66060 -109.73 -461.96 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain D : 0.86 3D Compatibility (PKB) : -109.73 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_1N1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N1Q-query.scw PDB file : Tito_Scwrl_1N1Q.pdb: