Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEVKSYLKEYGVNVR 3IIP Chain:C ((3-250)) -LAKRIDAALIVKDGRVVEGSNFENLRDSGDPVELGKFYSEIGIDELSFWDITASVEKRKTMLELVEKVAEQIDIPFTVGGGIHDFETASELILRGADKVEINTAAVENPSLITQIAQTFGSQAVVVYIAAK--RVDGEFMVFTYSGTKNTGILLRDWVVEVEKRGAGEIVLGSIDRLGTKSGYDTEMIRFVRPLTTLPIIAHRGAGKMEHFLEAFLAG-ADAAKADSVFHFREIDVRELKEYLKKHGVNVR

General information: TITO was launched using: RESULT: Template: 3IIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1410 -146873 -104.16 -592.23 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -104.16 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3IIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IIP-query.scw PDB file : Tito_Scwrl_3IIP.pdb: