Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVMECQTYEELSQIAARITADTIKEKPDAVLGLATGGTPEGTYRQLIRLHQTENLSFQNITTVNLDEYAGLSSDDPNSYHFYMNDRFFQHIDSKP-SRHFIPNGNADDLEAECRRYEQLVDSLGDTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQANARYFPSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASALHLHSDVTVLIDREAASLRP 2RI0 Chain:B ((2-232)) MKTIKVKNKTEGSKVAFRMLEEEITFGAK-TLGLATGSTPLELYKEIRESH----LDFSDMVSINLDEYVGLSADDKQSYAYFMKQNLFA---AKPFKKSYLPNGLAADLAKETEYYDQILAQY-PIDLQILGIGRNAHIGFNEPGTAFSSQTHLVDLTPSTIAANSRFFEKAEDVPKQAISMGLASIMSAKMILLMAFGEEKAEAVAAMVKGPVTEEIPASILQTHPKVILIVDEKAGA---

General information: TITO was launched using: RESULT: Template: 2RI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1276 -45252 -35.46 -196.75 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -35.46 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_2RI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RI0-query.scw PDB file : Tito_Scwrl_2RI0.pdb: