Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVITYGTFDLLHWGHIKLLERAKQL--GDYLVVAISTDE-------FNLQKQKKAYHSYEHRKLILETIRYVDEVIPEKNWEQKKQDIIDHNIDVFVMGDD----WEGKFDFLKDQCEV-VYLP--RTEGISTTKIKEEIAGL 4MVD Chain:F ((98-228)) --RVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQH-------CRYVDEVVRNAPWTLTPEFLAEHRID-FVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDI-------

General information: TITO was launched using: RESULT: Template: 4MVD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 482 -5020 -10.41 -44.82 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain F : 0.75 3D Compatibility (PKB) : -10.41 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4MVD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MVD-query.scw PDB file : Tito_Scwrl_4MVD.pdb: