Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MRKKELFDFTNITPKLFTELRVADKTVLQSF-----NFDEKNHQIYTTQVASG-LGKDNTQSYRITRLSLEGLQLDSMLLKHGGHGTNIGIENRNGTIYIWSLYDKPNETDKSELVCFPYKAGATLDENSKELQRFSNMPFDHRVT--------PALDMKNRQLA-IRQ-YDTKNNNNKQWVTIFNLD-----DAIANKNNPLYT----INIPDELHYLQGFFLDDGYLYWYTGDTNSK---SYPNLITVFDSDNKIVLQKEITVG------KDLSTRYENNFREPEGICMYTNPETGAKSLMVGITSGKEGNRISRIYAY-H-SYENFMNHVPMLRSP-------LLKTVGHQDTPPERFQPFIQTFILEYNAQ-NKKWMVPTSGYLPSYTS-NLVRNITINADGNLQVTLNERYISLLHQSIEGDFRLKQKDIRM-GSWYFAGGEKSNVLE-IGFM-KGSTKIRPDDAAISNASRMSIFMIVADKIEV----------------------------- 5BP8 Chain:A ((6-520)) APREAEAAALLAATVADPWGLVAPSVYDTARLVSLAPWLDGHRERLGYLAKEQNQDGSWGAPDGYGLVPTLSAVEALLTELARTDSGAPHLSPDDLAAACADGLGALRDGLLAGPVPDTIGVEFVAPSLLADINTRLAALTEQAPGKLGAWSGTTLTSPAPDLDGALLAGVREMTEQAPLPEKLWHTLEAVTRDGTRGARPHEGAPPHNGSVGCSPAATAAWLGAAPDPAAPGVAYLRDVQARFGGPVPSITPIVYFEQAWVLNSLAASGLRYEAPAALLDSLEAGLTD-EGIAAAPGLPSDSDDTAAVLFALAQHGRTHRPDSLMHFRRDGYFSCFGVERTPSTSTNAHILEALGHHVTVRPDDAGRYGAEI-----RMISDWLLDNQ--LPDGSWMDKWHASPYYATACCALALAEFGGPSARAAVDRAAAWALATQRADGSWGRWQGTTEETAYMVQLLMRTRTPGSPGTVARSAARGCDALLAHDDPASYPGLWHDKDIYAPVTVIRAARLAALALGGA

General information: TITO was launched using: RESULT: Template: 5BP8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2391 75742 31.68 174.52 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 31.68 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.087

(partial model without unconserved sides chains): PDB file : Tito_5BP8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BP8-query.scw PDB file : Tito_Scwrl_5BP8.pdb: